Selected Publications

Jang E*, Ho TWW*, Brumell J, Lefebvre F, Wang C, Lee WL. IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a. Arterioscler Thromb Vasc Biol. 2024 Jul 11. doi: 10.1161/ATVBAHA.124.320940. Epub ahead of print. PMID: 38989581. 

Fung KYY, Ho TWW, Xu Z, Neculai D, Beauchemin CAA, Lee WL*, Fairn GD* (*co-corresponding authors).  Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding SR-B1. Journal of Lipid Research 2024 (in press)

Henry A, Lee WL. Unexpected Antiatherogenic Effect: Myeloid-Derived Growth Factor Inhibits LDL Transcytosis. Arteriosclerosis, Thrombosis, and Vascular Biology 2023;43:2115-2118.

Sanwal R, Mintsopoulos V, Ditmans M,  Lang A, Latreille E, Ghaffari S, Khosraviani N, Karshafian R, Leong-Poi H, Hwang DM, Brochard L, Goffi A,  Slutsky AS, Lee WL. Ultrasound-guided transfection of claudin-5 improves lung endothelial barrier function in lung injury without impairing innate immunity. American Journal of Physiology - Lung Cellular and Molecular Physiology 2023; Jun 13. doi: 10.1152/ajplung.00107.2023. 

Lee S, Schleer H, Park H, Jang E, Boyer M, Tao B, Gamez-Mendez A, Singh A, Folta-Stogniew E, Zhang X, Qin L, Xiao X, Xu L, Zhang J, Hu X, Pashos E, Tellides G, Shaul PW, Lee WL, Fernandez-Hernando C, Eichmann A, Sessa WC Genetic or therapeutic neutralization of ALK1 reduces LDL transcytosis and atherosclerosis in mice. Nat Cardiovasc Res (2023). https://doi.org/10.1038/s44161-023-00266-2 

Lee WL, Slutsky AS. A negative trial for vasoactive intestinal peptide in COVID-19-associated acute hypoxaemic respiratory failure. Lancet Respiratory Medicine 2023 (in press).

Guo X*, Khosraviani N*, Raju S, Singh J, Farahani NZ, Abramian M, Torres VJ, Howe KL, Fish JE, Kapus A, Lee WL. Endothelial ACKR1 is induced by neutrophil contact and down-regulated by secretion in extracellular vesicles. Frontiers in Immunology; 14 - 2023 | doi: 10.3389/fimmu.2023.1181016 

Joshi K, Sanwal R, Thu K, Tsai SSH, Lee WL.  Plug & Pop: A 3D-Printed, Modular Platform for Drug Delivery using Clinical Ultrasound and Microbubbles. Pharmaceutics 2022;14(11):2516. 

Jang E., Ghaffari S., Lee W.L. (2022) Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF). In: Heit B. (eds) Fluorescent Microscopy. Methods in Molecular Biology, vol 2440. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2051-9_7

Sanwal R, Joshi K, Ditmans M, Tsai SSH, Lee WL. Ultrasound and Microbubbles for Targeted Drug Delivery to the Lung Endothelium in ARDS: Cellular Mechanisms and Therapeutic Opportunities. Biomedicines. 2021; 9(7):803. https://doi.org/10.3390/biomedicines9070803 

Latreille E, Lee WL. Interactions of Influenza and SARS-CoV-2 with the Lung Endothelium: Similarities, Differences, and Implications for Therapy. Viruses 2021, 13(2), 161; https://doi.org/10.3390/v13020161 

Ghaffari S, Jang E, Naderinabi F, Sanwal R, Khosraviani N, Wang C, Steinberg B, Goldenberg N, Ikeda J, Lee WL. Endothelial HMGB1 is a critical regulator of LDL transcytosis via a SREBP2-SR-BI axis. Arteriosclerosis, Thrombosis, and Vascular Biology 2020. PMID 33054399 

Accompanying editorial
 

Jang E, Robert J, Rohrer L, von Eckardstein A, Lee WL. Transendothelial transport of lipoproteins. Atherosclerosis 2020; Sep 25;S0021-9150(20)30539-6. PMID 33032832

Sanwal R, Khosraviani N, Advani SL, Advani A, Lee WL. The endothelial barrier is not rate-limiting to insulin action in the myocardium of male mice. Endocrinology 2020 Feb 27; PMID 32103251.

Filewod C, Lee WL. Inflammation without vascular leakage – science fiction no longer? American Journal of Respiratory and Critical Care Medicine 2019;200(12):1472-1476. 

Lubkin A, Lee WL*, Alonzo F 3rd, Wang C, Aligo J, Keller M, Girgis NM, Reyes-Robles T, Chan R, O’Malley A, Buckley P, Vozhilla N, Vasquez M, Su J, Sugiyama M, Yeung ST, Coffre M, Bajwa S, Chen E, Martin P, Kim SY, Loomis C, Worthen GS, Shopsin B, Khanna KM, Weinstock D, Lynch AS, Koralov SB, Loke P, Cadwell K, and Torres VJ.* (*co-corresponding authors). Staphylococcus aureus leukocidins target endothelial DARC to cause lethality in mice. Cell Host Microbe 2019;25:1-8. 

Raheel H*, Ghaffari S*, Khosraviani N, Mintsopoulos V, Auyeung D, Wang C, Kim YH, Mullen B, Sung H-K, Ho M, Fairn G, Neculai D, Febbraio M, Heit B, Lee WL. CD36 mediates albumin transcytosis by dermal but not lung microvascular endothelial cells - role in fatty acid delivery. American Journal of Physiology - Lung Cellular and Molecular Physiology 2019;316(5):L740-750.
Accompanying editorial: https://www.physiology.org/doi/abs/10.1152/ajplung.00104.2019
 

Ghaffari S, Naderi Nabi F, Sugiyama MG, Lee WL. Estrogen inhibits LDL transcytosis by human coronary artery endothelial cells via GPER and SR-BI. Arteriosclerosis, Thrombosis, and Vascular Biology 2018;38:2283-2294.
Accompanying editorial: https://www.ahajournals.org/doi/10.1161/ATVBAHA.118.311620
 

Sugiyama MG*, Mintsopoulos V*, Raheel H, Goldenberg NM, Batt JE, Kuebler WM, Brochard L, Leong-Poi H, Karshafian R, Lee WL. Lung ultrasound and microbubbles enhance aminoglycoside efficacy and delivery to the lung in the lung in E. coli-induced pneumonia and ARDS. American Journal of Respiratory and Critical Care Medicine 2018;198(3):404-408; PMID 29638143.
 

Filewod N, Batt J, Kapus A, Szaszi K, Fairn GD, Slutsky AS, Lee WL. Should basic science matter to clinicians? The Lancet 2018 Feb;391:410-412. 

Fung K, Fairn G, Lee WL. Transcellular vesicular transport in epithelial and endothelial cells: Challenges and opportunities. Traffic 2017.
 

Fung KY, Wang C, Nyegaard S, Heit B, Fairn GD, Lee WL. SR-BI Mediated Transcytosis of HDL in Brain Microvascular Endothelial Cells Is Independent of Caveolin, Clathrin, and PDZK1. Frontiers in Physiology. 2017 Oct 30;8:841.
 

Kraehling JR, Chidlow JH, Rajagopal C, Sugiyama MG, Fowler JW, Lee MY, Zhang X, Ramírez-Hidalgo CM, Park EJ, Tao B, Chen K, Kuruvilla L, Larriveé B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle MW, Herz J, Williams KJ, Eichmann A, Lee WL, Fernández-Hernando C, Sessa WC. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nature Communications 2016 Nov;7:13516.
 

Sugiyama M*, Gamage A*, Zyla R, Armstrong SM, Wang C, Advani S, Advani A, Lee WL. Influenza infection induces platelet-endothelial adhesion that contributes to lung injury. Journal of Virology 2016 Feb;90(4):1812-23. PMID: 26637453
 

Lee WL, Klip A. Endothelial transcytosis of insulin: Does it contribute to insulin resistance? Physiology (Bethesda) 2016 Sept; 31(5):336-45. PMID 27511460.
 

Steinberg BE, Goldenberg NM, Fairn GD, Kuebler WM, Slutsky AS, Lee WL. Is basic science disappearing from medicine? The decline of biomedical research in the medical literature. The Faseb Journal 2016 Feb;30(2):515-8. PMID: 26467794

Armstrong S, Sugiyama M, Fung YY, Gao Y, Wang C, Levy AS, Azizi P, Roufaiel M, Zhu S-N, Neculai D, Yin C, Bolz S-S, Seidah N, Cybulsky M, Heit B, Lee WL. A novel assay uncovers an unexpected role for SR-BI in LDL transcytosis. Cardiovascular Research 2015 Nov 1;108(2):268-77.
 

Sugiyama MG, Armstrong SM, Wang C, Hwang D, Leong-Poi H, Advani A, Advani S, Szaszi K, Tabuchi A, Kuebler WM, Van Slyke P, Dumont DJ, Lee WL. The Tie2-agonist Vasculotide rescues mice from severe influenza virus infection. Scientific Reports 2015;5: 11030.
 

Pillon N, Azizi PM, Li YE, Liu J, Wang C, Chan KL, Hopperton KE, Bazinet RP, Heit B, Bilan PJ, Lee WL*, Klip A*. Palmitate-induced inflammatory pathways in human adipose microvascular endothelial cells promotes monocyte adhesion and impairs insulin transcytosis. American Journal of Physiology - Endocrinology and Metabolism 2015 Jul 1;309(1):E35-44. PMID: 25944880

Wang C*, Armstrong SM*, Sugiyama MG, Tabuchi A, Krauszman A, Kuebler WM, Mullen B, Advani S, Advani A, Lee WL. Influenza primes human lung microvascular endothelium to leak upon exposure to Staphylococcus aureus. American Journal of Respiratory Cell and Molecular Biology 2015 Oct;53(4):459-70.
 

Azizi PM, Zyla RE, Guan S, Wang C, Liu J, Bolz S-S, Heit B, Klip A, Lee WL. Clathrin-dependent entry and vesicle-mediated exocytosis define insulin transcytosis across microvascular endothelial cells. Molecular Biology of the Cell 2015 Feb 15;26(4):740-50. doi: 10.1091/mbc.E14-08-1307. Epub 2014 Dec 24. 

Goldenberg NM, Leligdowicz A, Slutsky AS, Friedrich JO, Lee WL. Is nosocomial infection really the major cause of death in sepsis? Critical Care 2014, 18:540  doi:10.1186/s13054-014-0540-y. PMID: 25672933.
 

Armstrong SM, Mubareka S, Lee WL.  The lung microvascular endothelium as a therapeutic target in severe influenza.  Antiviral Research 2013 Aug;99(2):113-8. doi: 10.1016/j.antiviral.2013.05.003.
 

Armstrong SM, Wang C, Tigdi J, Si X, Dumpit C, Charles S, Gamage A, Moraes, TJ, & Lee WL. Influenza Infects Lung Microvascular Endothelium Leading to Microvascular Leak: Role of Apoptosis and Claudin-5. Plos One 2012, 7(10):e47323.  

Armstrong SM, Khajoee V, Wang C, Wang T, Tigdi J, Yin J, Kuebler WM, Gillrie M, Davis SP, Ho M, Lee WL. Co-regulation of transcellular and paracellular leak across microvascular endothelium by dynamin and Rac. The American Journal of Pathology 2012, 180(3), 1308–23. 

Petruzziello-Pellegrini TN, Yuen DA, Page AV, Patel S, Soltyk AM, Matouk CC, Wong DK, Turgeon PJ, Fish JE, Ho JJ, Steer BM, Khajoee V, Tigdi J, Lee WL, Motto DG, Advani A, Gilbert RE, Karumanchi SA, Robinson LA, Tarr PI, Liles WC, Brunton JL, Marsden PA. The CXCR4 / CXCR7 / SDF-1 pathway contributes to the pathogenesis of Shiga toxin – associated hemolytic uremic syndrome in humans and mice.  Journal of Clinical Investigation 2012, 122(2):759-76.
 

Goldenberg NM, Steinberg BE, Slutsky AS, Lee WL. Broken barriers: a new take on sepsis pathogenesis. Science Translational Medicine 2011, 3(88), 88ps25.
 

Lee WL, Slutsky AS. Sepsis and Endothelial Permeability.  N Engl J Med 2010, 363(7):689-91.